3t3 mouse embryo fibroblast cell line (ATCC)

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ATCC 3t3 mouse embryo fibroblast cell line
3t3 Mouse Embryo Fibroblast Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+embryo+fibroblast+nih+3t3+cells/NIH%2F3T3/pmc12948079-136-19-25
Average 99 stars, based on 13210 article reviews

3t3 mouse embryo fibroblast cell line - by Bioz Stars, 2026-09

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Article Title: Graphene Enhances Actin Filament Assembly Kinetics and Modulates NIH-3T3 Fibroblast Cell Spreading
Article Snippet: Mouse embryo fibroblast NIH-3T3 cells were purchased from American Type Culture Collection (ATCC; Rockville, MD, USA).

Article Title: Type II Collagen from Cartilage of Acipenser baerii Promotes Wound Healing in Human Dermal Fibroblasts and in Mouse Skin
Article Snippet: Mouse embryo fibroblast NIH-3T3 cells and human keratinocytes HaCaT cells were purchased from American Type Culture Collection (Rockville, MD, USA) and grown in DMEM including 2 mM glutamine (Gibco BRL, Grand Island, NY, USA), 10% ( v / v ) fetal bovine serum, and 1% penicillin/streptomycin (10,000 units of penicillin/mL and 10 mg streptomycin/mL).

Article Title: Landscape of gene fusions in hormone receptor-positive breast cancer reveals ADK fusions as drivers of progression and potential therapeutic targets
Article Snippet: Mouse embryo fibroblast NIH-3T3 cells were also purchased from the ATCC.

Transfection:

Article Title: Inhibition of PTTG1 expression by microRNA suppresses proliferation and induces apoptosis of malignant glioma cells.
Article Snippet: .. Transfected cells (1x105 cells; 100 μl/well) were added to the top chamber, while 600 μl chemokines prepared from mouse embryo fibroblast NIH/3T3 cells (American Type Culture Collection, Manassas, VA, USA) (14) were added to the bottom chamber. .. The Transwell plate was incubated at 37 ̊C with 5% CO2 for 36 h. The plate was cleaned to remove immobile cells, and was fixed in 100% methanol (Seebio Biotech, Inc., Shanghai, China) for 10 min, followed by Giemsa staining (Seebio Biotech, Inc.) for 15 min.

Article Title: Inhibition of PTTG1 expression by microRNA suppresses proliferation and induces apoptosis of malignant glioma cells
Article Snippet: .. Transfected cells (1×10 5 cells; 100 μl/well) were added to the top chamber, while 600 μl chemokines prepared from mouse embryo fibroblast NIH/3T3 cells (American Type Culture Collection, Manassas, VA, USA) ( 14 ) were added to the bottom chamber. .. The Transwell plate was incubated at 37°C with 5% CO 2 for 36 h. The plate was cleaned to remove immobile cells, and was fixed in 100% methanol (Seebio Biotech, Inc., Shanghai, China) for 10 min, followed by Giemsa staining (Seebio Biotech, Inc.) for 15 min.

Cell Culture:

Article Title: Identification of adipocyte plasma membrane-associated protein as a novel modulator of human cytomegalovirus infection
Article Snippet: Human retinal pigment epithelia cells ARPE-19 (ATCC, CRL-230) were cultured in DMEM/F12 (50/50) plus 10% FBS. .. Human MRC-5 (ATCC, CCL-171) embryonic lung fibroblast cells, dog kidney epithelial cells MDCK (ATCC, CCL-34) and mouse embryo fibroblast NIH/3T3 cells (ATCC, CRL-1658) were cultured in DMEM plus 10% FBS. ..

Article Title: Identification of adipocyte plasma membrane-associated protein as a novel modulator of human cytomegalovirus infection.
Article Snippet: Cells, virus strains and reagents Human retinal pigment epithelia cells ARPE-19 (ATCC, CRL-230) were cultured in DMEM/ F12 (50/50) plus 10% FBS. .. Human MRC-5 (ATCC, CCL-171) embryonic lung fibroblast cells, dog kidney epithelial cells MDCK (ATCC, CCL-34) and mouse embryo fibroblast NIH/3T3 cells (ATCC, CRL-1658) were cultured in DMEM plus 10% FBS. ..

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